Journal: Aging (Albany NY)

Article Title: 3,4,5-Tricaffeoylquinic acid induces adult neurogenesis and improves deficit of learning and memory in aging model senescence-accelerated prone 8 mice

doi: 10.18632/aging.101748

Figure Lengend Snippet: The effect of 3,4,5-triCaffeoylquinic acid (TCQA) on intracellular Ca 2+ levels, mitochondrial function, and intracellular reactive oxygen species (ROS) levels at very early phase of human neural stem cell (hNSCs) differentiation. hNSCs were pre-treated with Fluo4 AM for 30 min followed by treatment with growth medium or differentiation medium with or without TCQA 10 μM for 1–30 min. Time after differentiation and TCQA treatment, intracellular Ca 2+ level was detected by measurement of fluorescence intensity ( A ). hNSCs were treated with growth medium or differentiation medium with or without 10 μM TCQA for 30–180 min. TCQA was treated with rhodamine 123 and detected mitochondrial function by measuring the fluorescence intensity ( B ). Intracellular ROS levels were detected by measuring fluorescence intensity of DCF oxidized by ROS. hNSCs were pre-treated with DCFH-DA for 1 h followed by treatment with growth medium or differentiation medium with or without 10 μM TCQA for 15–180 min ( C ). Data was set as % of undifferentiated control. Data were presented as mean ± SD. * P < 0.01, ** P < 0.01 Compared with undifferentiated control cells.

Article Snippet: Therefore, the effects of TCQA on intracellular ROS level was determined using the OxiSelect Intracellular ROS assay kit (Cosmo Bio, Japan) following the manufacturer’s procedures. hNSCs (2.5 × 10 4 cells/cm 2 ) were seeded in black clear bottom 96-well plates and were incubated in medium with TCQA for 1 h. The cells were then incubated with 10 μM DCFH-DA at 37°C for 1 h and washed with PBS.

Techniques: Fluorescence, Control